| Title | Non-targeted analyses of animal plasma: Betaine and choline represent the nutritional and metabolic status |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Katayama, K. , Sato T. , Arai T. , Amao H. , Ohta Y. , Ozawa T. , Kenyon P R. , Hickson R E. , and Tazaki H. |
| Journal | Journal of Animal Physiology and Animal Nutrition |
| Volume | 97 |
| Issue | 1 |
| Pagination | 119 - 125 |
| Date Published | 2013 |
| ISBN Number | 09312439 (ISSN) |
| Keywords | animal , Animalia , Animals , article , betaine , blood , cattle , chemistry , chicken , Chickens , choline , Diabetes Mellitus, Experimental , experimental diabetes mellitus , female , Institute for Cancer Research mouse , Liquid chromatography , male , Mass spectrometry , metabolism , Metabolomics , Metabonomics , Mice , Mice, Inbred ICR , mouse , Mus , plasma , Postprandial Period , postprandial state , Pregnancy |
| Abstract |
Simple liquid chromatography-mass spectrometry (LC-MS) was applied to non-targeted metabolic analyses to discover new metabolic markers in animal plasma. Principle component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) were used to analyze LC-MS multivariate data. PCA clearly generated two separate clusters for artificially induced diabetic mice and healthy control mice. PLS-DA of time-course changes in plasma metabolites of chicks after feeding generated three clusters (pre- and immediately after feeding, 0.5-3h after feeding and 4h after feeding). Two separate clusters were also generated for plasma metabolites of pregnant Angus heifers with differing live-weight change profiles (gaining or losing). The accompanying PLS-DA loading plot detailed the metabolites that contribute the most to the cluster separation. In each case, the same highly hydrophilic metabolite was strongly correlated to the group separation. The metabolite was identified as betaine by LC-MS/MS. This result indicates that betaine and its metabolic precursor, choline, may be useful biomarkers to evaluate the nutritional and metabolic status of animals. © 2011 Blackwell Verlag GmbH. |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84872977319&partnerID=40&md5=b60e0770a824bf0bd0209529e6792d2f |
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Non-targeted analyses of animal plasma: Betaine and choline represent the nutritional and metabolic status
About
The Beef research team has access to over 1000 hectares of university land near the campus, which provides unsurpassed opportunities to conduct research into pastoral beef production and health. A number of projects are conducted in collaboration with other leading research groups in New Zealand and throughout the world, and the group regularly consults with industry professionals and cattle producers. Our aim is to undertake quality research consistent with the needs of the New Zealand Beef Industry and to advance scientific knowledge of beef production, welfare, health and biology.
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Professor Steve Morris (Leader)
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Dr Rebecca Hickson
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